Proximate Composition Qualitative And Quantitative Phytochemical Analysis Of Delonlx Regia Leaves

ABSTRACT

Delonixregia is a traditionally medicine that has been used in various ailments such as chronic fever, antimicrobial, constipation, inflammation, arthritis, hemoplagia, piles, boils, pyorrhea, scorpion bite, bronchitis, asthma and dysmenorrhoea. However, there is little experimental evidence for its traditional use. In this review an attempt has been made to gather and compile the scattered traditional information along with the experimental evidence on the beneficial properties of Delonixregia. The plant shows diverse therapeutic prospective such as antifungal, antibacterial, antioxidant, antiemetic, larvicidal, hepatoprotective, anti-diarrhoeal, anti-inflammatory, antimalarial, anthelmintic, antiarthritic, wound healing and anticarcinogenic potential. It possess copious phytochemicals, viz. saponins, alkaloids, carotene, hydrocarbons, phytotoxins, flavonoids, tannins, steroids, carotenoids, galactomannon, lupeol, β-sitosterol, terpenoids, glycosides and carbohydrates, in leaves, flowers, bark and roots. Though DelonixregiaRaf. has been widely used in traditional medicines in various ailments, yet studies need to be conducted to explore the potential phyto-constituents of this plant for the prevention of various other diseases and to further unravel, characterize, patent and commercialize the protective components from different parts of this plant for the benefit of humans.

 TABLE OF CONTENTS

 TITLE PAGE

APPROVAL PAGE

DEDICATION

ACKNOWLEDGEMENT

ABSTRACT

TABLE OF CONTENT

CHAPTER ONE

• INTRODUCTION
• BACKGROUND OF THE STUDY
• PROBLEM STATEMENT
• AIM AND OBJECTIVES OF THE STUDY
• RESEARCH QUESTIONS
• SIGNIFICANCE OF THE STUDY
• LIMITATION OF THE STUDY
• DEFINITION OF TERMS

CHAPTER TWO                                                

2.0    LITERATURE REVIEW

2.1      DELONIX REGIA

2.2     HISTORICAL BACKGROUND OF DELONIX REGIA

2.3     OCCURANCE OF DELONIX REGIA

2.4     BOTANICAL DISCRIPTION

2.5      USES OF DELONIX REGIA

2.6    MEDICINAL & TRADITIONAL USES

2.7    PHYTOCHEMICAL CONSTITUENTS

2.8    ISOLATED COMPOUNDS

2.9    DISTRIBUTION OF DELONIX REGIA

2.10  FORAGE MANAGEMENT OF DISTRIBUTION OF DELONIX REGIA

2.11  ENVIRONMENTAL IMPACT

CHAPTER THREE

3.0      MATERIALS AND METHOD

3.1      STUDY AREA

3.2     PLANTCOLLECTIONANDPREPARATION

3.3      MEASUREMENTS

3.3.1  PROXIMATEANALYSIS

3.4        MINERALANALYSIS

3.5    STATISTICALANALYSIS

CHAPTER FOUR

4.0    RESULT AND DISCUSSION

4.1   PROXIMATE COMPOSITIONOFDRLAND DSR

4.2 MINERAL ANALYSIS OF DRL AND DSR

CHAPTER FIVE

• CONCLUSIONS

CHAPTER ONE

1.0                                                        INTRODUCTION

1.1                                           BACKGROUND OF THE STUDY

Medicinal plants are rich source of novel drugs that forms the ingredients in traditional system of medicine, modern medicines, pharmaceutical intermediates and lead compounds in synthetic drugs [1]. The reason for using them as medicine lies in the fact that they contain chemical components of therapeutic value [2]. These compounds are naturally occurring in the medicinal plants, leaves, vegetables and roots that have defense mechanism and protect from various diseases. The medicinal value of plants lies in some chemical substances (usually secondary metabolites) that produce a definite physiological action as the human body. In recent times focus on plant research has increased all over the world and a large body of evidence has collected to show immense potential of medicinal plants used in various traditional systems [3] including treatment against hepatocellular carcinoma [4].

Nature has been a source of wide diversity of medicinal plants and mankind has used many species for centuries since time immemorial for their benefit. The medicinal property of the plants has been exploited for the cure of several ailments [4]. There has been an increasing interest worldwide on therapeutic values of natural products from plants due to  disenchantment with modern synthetic drug [1]. Herbal medicine has proved efficacious and potent in the treatment of many chronic diseases that orthodox medicine cannot cure. Plants have the ability to synthesize a wide variety of chemical compounds that are used to perform important biological functions, and to defend against attack from predators such as insects, fungi and herbivorous mammals. Antibiotics were discovered to provide the source for the therapy of microbial infections. Excessive use of antibiotics has become the major factor for the emergence and dissemination of multidrug resistant strains [16]. Numerous aromatic spicy and medicinal plants have been examined for their antioxidant potential [3]. Many plant components are now synthesized and analyzed in large laboratories, for example vincristine (antitumor drug) and ephedrine (bronchodilator) used to decrease respiratory congestion were well originally discovered through research on medicinal plants [9]. In general people with low income such as farmers, people of small isolated villages and native communities use folk medicine for the treatment of common infections [8]. Infectious diseases caused by these multidrug resistant strains remain the leading cause of death. Thus people are turning their attention to alternative novel antimicrobial agents to combat such pathogens [5,10].

Phytochemicals are biologically active compounds derived from plant physiological processes and are, responsible for conferring colour, flavor, smell, texture and several biological properties including antimicrobial property to the plants [11]. India is rich in all the three levels of bio diversities including species diversity, genetic diversity and habitat diversity. Therefore, after search through wide literature for medicinal plants bearing antimicrobial properties the plants Delonixregia, Cassia alata, and Murrayakoenigii plants were selected to further screen their phytochemical properties. The aim to analyze the phytochemical properties of the concerned plant (Delonixregia) was to validate their use in folk medicine. At the end of this work, the proximatecomposition,quantitative and qualitative phytochemical constituents of Delonixregia leaves shall be analyzed and discussed.

1.2                                              STATEMENT OF PROBLEM

Traditional medicine is widely used almost everywhere in the world most especially in Africa which Nigeria is one of the countries in Africa that values and use traditional medicine. However, despite the popularity of traditional medicine in Nigeria, there are still high number of people that doesn’t belief in the efficacy of traditional medicine. This work was made to gather and compile the scattered traditional information along with the experimental evidence on the beneficial properties of Delonixregia  which can make and convince people using traditional medicine.

1.3                                         AIM AND OBJECTIVES OF THE STUDY

The main aim of this study is to determine the proximate composition qualitative and quantitative phytochemical analysis of delonlxregia leaves.

The objectives of the study are:

1. To determine extent is Delonixregia useful
2. To determine sicknesses that Delonixregia cures

• To check if there any side effect of using Delonixregia in treating sicknesses

1.4                                                   RESEARCH QUESTION

1. To what extent is Delonixregia useful?
2. What are the sicknesses that Delonixregia cures?
3. Is there any side effect of using Delonixregia in treating sicknesses?

1.5                                                SIGNIFICANCE OF STUDY

As a student of this department, this study has exposed me to the uses, application, and chemical ingredient of Delonixregia.This study has also help me toinvestigatetheproximatecomposition,quantitative and qualitative phytochemical constituents of Delonixregia.

1.6                                                SCOPE AND LIMITATION

Medicinal plants have bioactive compounds which are used for curing of various human diseases and also play an important role in healing. Secondary constituents contain Alkaloids, Flavonoids, Phenol, Saponine, Steroids and Tannins. Medicinal plants have anticancer, antimicrobial, antidiabetic, antidiuretic and anti-inflammation activities. There are different types of medicinal plants which cures different types of diseases but in this study we are only focusing on Delonixregia which is locally available in all region of Nigeria. The leaves of the selected medicinal plants were washed, air dried and then powdered. The methanolicextract of leaves samples were used for the phytochemical analysis to find out the quantitative and quantitative phytochemical constituents in the plants.

1.7                                                  DEFINITION OF TERMS

Most frequently used terms in this work are defined as below:

DELONIX REGIA:Delonixregia is a species of flowering plant in the bean family Fabaceae, subfamily Caesalpinioideae native to Madagascar. It is noted for its fern-like leaves and flamboyant display of orange-red flowers over summer.

PHYTOCHEMICALS: These are chemical compounds produced by plants, generally to help them thrive or thwart competitors, predators, or pathogens. Some phytochemicals have been used as poisons and others as traditional medicine.

MEDICINAL PLANT: This is a plant that is used with the intention of maintaining health, to be administered for a specific condition, or both, whether in modern medicine or in traditional medicine.

ANTIMICROBIAL: An antimicrobial is an agent that kills microorganisms or stops their growth. Antimicrobial medicines can be grouped according to the microorganisms they act primarily against. For example, antibiotics are used against bacteria and antifungals are used against fungi.

CHAPTER FIVE

CONCLUSION

The study was a scientific validation that Delonix regia contains essential minerals, nutrients and some chemical substances that produce definite physiological action on the body of human and animals. Medicinal plants are safe, cheap and effective and are could also be used in future to bridge the gap between food safety and production in livestock.